Optical HREM Applications

HREM offers a unique approach to imaging embryos of varied species from the easier Zebrafish to the denser Mouse and Chick embryos. It offers this with no distortion, missing sections or clearing required.

• Optical HREM systems offer high resolution on larger volumes, down to 1 micron in the axial direction. Ideal for visualising blood vessels and similar features

• Quantify organ volumes of mice and similar species, as well as compare time points such as E9.5 vs E12.5

Image whole mice (or any species) organs up to 15/20mm (greater, sample dependent) allowing for imaging of these denser areas to be visualised ex-Vivo or in-Situ. Combine HREM with multi-fluoresce for experimental staining and exploration.

• 3D Kidney, Brain, Lung, Liver and Spleen in one high resolution dataset

• Understand spatial relationships that 2D slices cannot visually explain

Image whole cardiac structures with close to histology resolution, with the advantage of 3D analysis.

• Measure chamber volumes with accuracy and segment manually as well as wall thickness and valve morphology

• Image the heart within the structure at higher resolution with scanning

• Ideal for the study of congenital heart defects, angiogenesis and more heart applications

Craniofacial imaging requires imaging of extremely dense structures including bone, HREM handles bone extremely well. For imaging head, face, jaw and brain regions in smaller models such as mice.

• Captures hard and soft tissue together with bone, cartilage, skin, nerves, and vessels

• Ideal for cleft palette, craniosynostosis and further development studies

• Quantitative measurements for jaw angles, sinus volumes and brain/skull alignment

Plant structures often prove difficult to image, due to the density, HREM offers a solution for this. With clear, even contrast on samples up to 20mm (more, sample dependent) allowing full reconstruction of developing plants, seeds, and roots.

• HREM has been used to visualise seed structure, fruit peel oil cavities, stem vascular bundles and growing in this sector

• Easily compare mutants and wild type

• Ideal for quantitative studies for cavity density, wall thickness or overall morphology

Image the complex structures of inspects such as drosophila (fruit fly) for imaging of both cuticle and soft tissue without registration.

• Ideal for imaging mutants, developmental stages and transgenic lines

• No need for dissection, image whole mount at resolutions down to 1 micron with clear contrast